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Allele-specific oligonucleotide hybridization

Related Terms

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Background

  • An allele-specific oligonucleotide (ASO) is a short, single-strand deoxyribonucleic acid (DNA) molecule, usually 15-21 nucleotides, or bases, in length.
  • DNA makes up the genetic "blueprint" that contains all the information necessary to make a living being. The unique twisted ladder shape of DNA is called a double helix. The sides of the double helix are made of alternating sugar and phosphate molecules. The "rungs" of the "ladder" are made of small molecules called bases. There are four different types of these bases in DNA: adenine, thymine, cytosine, and guanine. These bases are arranged in a unique order, or sequence, in the DNA. Because they contain nitrogen, these molecules are sometimes called nitrogen bases. DNA provides instructions for the production of a molecule called mRNA (messenger ribonucleic acid), which then controls the production of protein encoded by the gene.
  • Alleles are variations of a single gene; each gene contains two alleles because one is inherited from each parent. A mutation occurs when the chemicals that make up an individual's genes are incorrectly deleted, added, or substituted.
  • An ASO is complementary, meaning that it pairs with the sequence of a variable target DNA. The base pairs in a DNA molecule bind to each other in a particular way. The adenine base is complementary to thymine and the cytosine to guanine. The sequence of the bases defines the information contained in the DNA. A large proportion of DNA is the same in all human beings, but there are some regions of DNA that show considerable variation among individuals in the population.
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Methods

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Research

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Implications

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Limitations

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Safety

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Future Research

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Author Information

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References

Natural Standard developed the above evidence-based information based on a thorough systematic review of the available scientific articles. For comprehensive information about alternative and complementary therapies on the professional level, go to www.naturalstandard.com. Selected references are listed below.

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The information in this monograph is intended for informational purposes only, and is meant to help users better understand health concerns. Information is based on review of scientific research data, historical practice patterns, and clinical experience. This information should not be interpreted as specific medical advice. Users should consult with a qualified healthcare provider for specific questions regarding therapies, diagnosis and/or health conditions, prior to making therapeutic decisions.