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Padlock probes

Related Terms

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Background

  • A padlock probe (PLP), a long oligonucleotide whose ends are complementary to an adjacent target sequence, is used to detect and identify a target molecule in a sample. PLP is used in genetic analysis techniques. Genetic analysis is performed to detect variations, or mutations, in the sequence of a gene, which may be associated with the development of certain diseases in humans, plants, and/or animals. Thus, detection of mutations may help in the early identification and prevention of these associated diseases. Padlock probes have a very good distinguishing quality (specificity), which makes them significantly advantageous over other conventional oligonucleotide probes.
  • Genes are the functional units of deoxyribonucleic acid (DNA) and are considered the building blocks of life because they provide instructions for all the cells in the body. Genes, which are located inside cells, control an organism's development and functions by instructing cells to make new molecules (usually proteins).
  • DNA is a long thread-like molecule made up of large numbers of nucleotides. Nucleotides are molecules composed of a nitrogen base, a 5-carbon sugar, and one or more phosphate groups. Long strands of nucleotides form nucleic acids, such as (DNA) and ribonucleic acid (RNA).
  • RNA is a single chain of nucleotides that contains ribose as its sugar (ribonucleotides). RNA determines the protein synthesis and transmission of genetic information.
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Methods

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Research

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Implications

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Limitations

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Safety

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Future Research

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Author Information

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References

Natural Standard developed the above evidence-based information based on a thorough systematic review of the available scientific articles. For comprehensive information about alternative and complementary therapies on the professional level, go to www.naturalstandard.com. Selected references are listed below.

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The information in this monograph is intended for informational purposes only, and is meant to help users better understand health concerns. Information is based on review of scientific research data, historical practice patterns, and clinical experience. This information should not be interpreted as specific medical advice. Users should consult with a qualified healthcare provider for specific questions regarding therapies, diagnosis and/or health conditions, prior to making therapeutic decisions.